High-pressure homogenization (HPH) can improve the functionalities of food proteins by forming soluble aggregates. This research aimed to determine the effects of thermal aggregation and high-pressure homogenization on the structural and emulsifying properties of kidney bean proteins.
اقرأ المزيدThe utility of high pressure refolding to remove and refold protein aggregates, enhance therapeutic proteins, and facilitate manufacturing improvements at industrial scales is summarized. Non-denaturing pressures of around 2000 bar are effective for eliminating and refolding protein aggregates and may be applicable in various …
اقرأ المزيدHigh pressure treatment can also be used to conduct selective PEGylation and protease cleavage reactions while minimizing protein aggregation. High pressure processes have been used in the food industry for over 50 years and large scale (300 L) systems are commercially available, enabling production of proteins on the kilogram scale.
اقرأ المزيدHigh-pressure homogenization (HPH) is a technique that impacts the aggregation of globular proteins. In this study, the effect of HPH (at a pressure of 30/50 MPa for three cycles) was investigated on the aggregation states and functional properties of insoluble commercial pea protein isolates (CPPI).
اقرأ المزيدThe pH method under pH 12 followed by high-pressure homogenization was found as the most efficient treatment in the reduction of protein aggregate sizes and transparency, improving soluble protein content and surface hydrophobicity.
اقرأ المزيدWe hypothesize that if the pressure effects on aggregates of non-native protein molecules are similar to those on native multimeric proteins, then there must exist a "pressure window" where pressure is high enough to solubilize aggregates, but still allow refolding to the native conformation.
اقرأ المزيدFunctionalising insoluble pea protein aggregates using high-pressure homogenisation: Effects on physicochemical, microstructural and functional properties. Author links open overlay panel Kian Siang Ong a b, Jie Hong Chiang a, Shaun Yong Jie Sim a, David Liebl c 1, Mufeeda Madathummal c, Christiani Jeyakumar Henry a d. Show …
اقرأ المزيدIn order to ascertain the compatibility of high proportions of garnet with the lunar seismic profiles we determined the sound velocities in an iron-rich garnet-rich lunar assemblage via high-pressure high-temperature in situ measurements at BL04B1 at SPring-8. Elastic wave velocity models of lunar rock aggregates constructed on the basis of ...
اقرأ المزيدThe band at ∼1647 cm −1 in Figure 2 (b) shows that applying pressure to these early aggregates results in their dissociation, as shown by the complete disappearance of the band around 1623 ... HHP measurement was performed in a multivessel high-pressure apparatus (Resato, The Netherlands). Pressure is …
اقرأ المزيدThe sintering behavior of olivine and olivine-basalt aggregates has been examined at temperatures near 1,300° C. Experimental factors contributing to rapid sintering kinetics and high-density, fine-grained specimens include: (i) the uniform dispersion of basalt throughout the specimen, (ii) a very fine, uniform particle size for the olivine powder, (iii) oxygen …
اقرأ المزيدConsequently, this study evaluated the effect of pasteurization methods (Holder pasteurization, high-temperature short-time (HTST), and high hydrostatic pressure (HHP)) of whole human milk (HM) on protein aggregates in skim milk and cream fractions.
اقرأ المزيدAccelerated carbonation is recognized as a possible way to improve the quality of recycled concrete aggregates (RCA) and thus increase the possibilities of their use for concrete manufacturing. This work is a contribution to exploring high-pressure carbonation as a possible technology for carbonation of cement-based RCA.
اقرأ المزيدHigh hydrostatic pressures (1-2 kbar), combined with low, nondenaturing concentrations of guanidine hydrochloride (GdmHCl) foster disaggregation and refolding of denatured and aggregated human growth hormone and lysozyme, and beta-lactamase inclusion bodies. One hundred percent recovery of properly …
اقرأ المزيدThe results obtained shed new light on asphaltene aggregate dynamics and brought new knowledge about the fundamental behavior of asphaltene in high-pressure CO 2 conditions. Read this article To access this article, please review the …
اقرأ المزيدHigh-pressure treatment induced a significant reduction in protein intrinsic fluorescence intensity suggesting irreversible partial protein unfolding, irrespective of pressure or pH. The highest pressure level (600 MPa) led to significantly higher …
اقرأ المزيدThe impacts of dynamic high-pressure (DHP) pretreatment and post-treatment (100 MPa) on the physicochemical and functional properties of whey protein isolate (WPI) aggregates formed by thermal treatment were investigated in this study. When WPI aggregates were formed by thermal treatment, the size of the aggregates …
اقرأ المزيدElectrical Conductivity of Ti‐Bearing Hydrous Olivine Aggregates at High Temperature and High Pressure Lidong Dai1,2 and Shun‐ichiro Karato2 1KeyLaboratoryofHigh ‐TemperatureandHigh PressureStudyoftheEarth'sInterior,InstituteofGeochemistry,Chinese Academy of Sciences, Guiyang, China, 2Department of Earth and Planetary Sciences, …
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